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cd68 immunostaining  (Agilent technologies)


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    Agilent technologies cd68 immunostaining
    Cd68 Immunostaining, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd68+immunostaining/10__1097_slash_pgp__0000000000000939-64-1-3
    Average 90 stars, based on 1 article reviews
    cd68 immunostaining - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Wright Stain:

    Article Title: HIV-1 gp120 compromises blood-brain barrier integrity and enhance monocyte migration across blood-brain barrier: implication for viral neuropathogenesis
    Article Snippet: .. These cells were identified as > 98% pure monocytes by Wright staining and CD68 immunostaining (at 1:50 dilution, Dako, Carpentaria, CA, USA). ..

    Article Title: HIV-1 gp120 induces cytokine expression, leukocyte adhesion, and transmigration across the blood-brain barrier: modulatory effects of STAT1 signaling
    Article Snippet: .. These cells were identified as >98% pure monocytes by Wright staining and CD68 immunostaining (at 1:50 dilution, Dako, Carpentaria, CA). ..

    Article Title: Differential mechanisms of inflammation and endothelial dysfunction by HIV-1 subtype-B and recombinant CRF02_AG Tat proteins on human brain microvascular endothelial cells: implications for viral neuropathogenesis.
    Article Snippet: .. Cells were identified as >98% pure monocytes by Wright staining and CD68 immunostaining (at 1:50 dilution, Dako, Carpentaria, CA). ..

    Article Title: Cross-Talk Between STAT1 and PI3K/AKT Signaling in HIV-1-Induced Blood–Brain Barrier Dysfunction: Role of CCR5 and Implications for Viral Neuropathogenesis
    Article Snippet: .. Cells were identified as >98% pure monocytes by Wright staining and CD68 immunostaining (at 1:50 dilution; Dako, Carpentaria, CA). .. Cells were identified as >98% pure monocytes by Wright staining and CD68 immunostaining (at 1:50 dilution; Dako, Carpentaria, CA).

    Immunostaining:

    Article Title: HIV-1 gp120 compromises blood-brain barrier integrity and enhance monocyte migration across blood-brain barrier: implication for viral neuropathogenesis
    Article Snippet: .. These cells were identified as > 98% pure monocytes by Wright staining and CD68 immunostaining (at 1:50 dilution, Dako, Carpentaria, CA, USA). ..

    Article Title: Evaluation of Histopathologic Alterations in First-trimester COVID-19-positive Pregnancies Ending in Abortion
    Article Snippet: This study aims to evaluate histopathologic alterations and the presence of chronic histiocytic intervillositis in first-trimester coronavirus-2019 (COVID-19)-positive pregnancies ending in abortion, compared with those at a similar gestational week and undergoing curettage before the pandemic.. This retrospective case-control study consisted of 9 patients who were infected with COVID-19 and undergoing curettage for abortion between April 2020 and January 2021.. The control group consisted of 34 patients with a similar gestational age who underwent curettage for abortion before August 2019.

    Article Title: HIV-1 gp120 induces cytokine expression, leukocyte adhesion, and transmigration across the blood-brain barrier: modulatory effects of STAT1 signaling
    Article Snippet: .. These cells were identified as >98% pure monocytes by Wright staining and CD68 immunostaining (at 1:50 dilution, Dako, Carpentaria, CA). ..

    Article Title: COBL , MKX and MYOC Are Potential Regulators of Brown Adipose Tissue Development Associated with Obesity-Related Metabolic Dysfunction in Children
    Article Snippet: Adipocyte size was measured after the fixation of freshly isolated adipocytes in osmium tetroxide (Science Services, Munich, Germany) using a Coulter counter (Multisizer III; Beckmann Coulter, Krefeld, Germany). .. The number of AT macrophages was assessed by CD68 immunostaining (M0718, DAKO, Glostrup, Denmark) of paraffin-embedded AT sections using the DAKO REALTM APAAP Immunocomplex system. ..

    Article Title: Differential mechanisms of inflammation and endothelial dysfunction by HIV-1 subtype-B and recombinant CRF02_AG Tat proteins on human brain microvascular endothelial cells: implications for viral neuropathogenesis.
    Article Snippet: .. Cells were identified as >98% pure monocytes by Wright staining and CD68 immunostaining (at 1:50 dilution, Dako, Carpentaria, CA). ..

    Article Title: Transcriptome Analyses of Adipose Tissue Samples Identify EGFL6 as a Candidate Gene Involved in Obesity-Related Adipose Tissue Dysfunction in Children
    Article Snippet: Adipocyte size was determined after fixation of freshly isolated adipocytes in osmium tetroxide (Science Services, Munich, Germany) using a Coulter counter (Multisizer III; Beckmann Coulter, Krefeld, Germany). .. The number of macrophages in AT was assessed by CD68 immunostaining (M0718, DAKO) using the DAKO REALTM APAAP Immunocomplex system in paraffin-embedded tissue sections and is given per 100 adipocytes. ..

    Article Title: Cross-Talk Between STAT1 and PI3K/AKT Signaling in HIV-1-Induced Blood–Brain Barrier Dysfunction: Role of CCR5 and Implications for Viral Neuropathogenesis
    Article Snippet: .. Cells were identified as >98% pure monocytes by Wright staining and CD68 immunostaining (at 1:50 dilution; Dako, Carpentaria, CA). .. Cells were identified as >98% pure monocytes by Wright staining and CD68 immunostaining (at 1:50 dilution; Dako, Carpentaria, CA).

    Immunohistochemical staining:

    Article Title: Evaluation of Histopathologic Alterations in First-trimester COVID-19-positive Pregnancies Ending in Abortion
    Article Snippet: This study aims to evaluate histopathologic alterations and the presence of chronic histiocytic intervillositis in first-trimester coronavirus-2019 (COVID-19)-positive pregnancies ending in abortion, compared with those at a similar gestational week and undergoing curettage before the pandemic.. This retrospective case-control study consisted of 9 patients who were infected with COVID-19 and undergoing curettage for abortion between April 2020 and January 2021.. The control group consisted of 34 patients with a similar gestational age who underwent curettage for abortion before August 2019.



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    Fig. 1. KUS121 attenuated atherosclerosis progression in vivo. (A) Scheme of experimental protocol to assess the effect of KUS121 on atherosclerosis progression. (B) Representative images of HE staining in aortic sinuses and (C) quantitative analysis of plaque areas (n = 15 per group). Scale bar: 200 μm. (D) Representative images of en face ORO staining of whole thoracic aorta and (E) quantification of atherosclerotic lesions in aortic arches and descending aortas (n = 17 per group). Scale bar: 2 mm. (F) Representative images of <t>CD68</t> staining in aortic sinuses and (G) quantification of <t>CD68-positive</t> areas in atherosclerotic plaques (n = 15 per group). Scale bar: 200 μm. (H) Quantification of the proportions of Chop-positive cells in different cell types and (I) representative images of Chop staining in the atherosclerotic plaques. Scale bar: 50 μm. All data is presented as mean ± SEM **p < 0.01, ***p < 0.001 by student’s t-test.
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    Image Search Results


    Fig. 1. KUS121 attenuated atherosclerosis progression in vivo. (A) Scheme of experimental protocol to assess the effect of KUS121 on atherosclerosis progression. (B) Representative images of HE staining in aortic sinuses and (C) quantitative analysis of plaque areas (n = 15 per group). Scale bar: 200 μm. (D) Representative images of en face ORO staining of whole thoracic aorta and (E) quantification of atherosclerotic lesions in aortic arches and descending aortas (n = 17 per group). Scale bar: 2 mm. (F) Representative images of CD68 staining in aortic sinuses and (G) quantification of CD68-positive areas in atherosclerotic plaques (n = 15 per group). Scale bar: 200 μm. (H) Quantification of the proportions of Chop-positive cells in different cell types and (I) representative images of Chop staining in the atherosclerotic plaques. Scale bar: 50 μm. All data is presented as mean ± SEM **p < 0.01, ***p < 0.001 by student’s t-test.

    Journal: Atherosclerosis

    Article Title: KUS121, a novel VCP modulator, attenuates atherosclerosis development by reducing ER stress and inhibiting glycolysis through the maintenance of ATP levels in endothelial cells.

    doi: 10.1016/j.atherosclerosis.2025.119223

    Figure Lengend Snippet: Fig. 1. KUS121 attenuated atherosclerosis progression in vivo. (A) Scheme of experimental protocol to assess the effect of KUS121 on atherosclerosis progression. (B) Representative images of HE staining in aortic sinuses and (C) quantitative analysis of plaque areas (n = 15 per group). Scale bar: 200 μm. (D) Representative images of en face ORO staining of whole thoracic aorta and (E) quantification of atherosclerotic lesions in aortic arches and descending aortas (n = 17 per group). Scale bar: 2 mm. (F) Representative images of CD68 staining in aortic sinuses and (G) quantification of CD68-positive areas in atherosclerotic plaques (n = 15 per group). Scale bar: 200 μm. (H) Quantification of the proportions of Chop-positive cells in different cell types and (I) representative images of Chop staining in the atherosclerotic plaques. Scale bar: 50 μm. All data is presented as mean ± SEM **p < 0.01, ***p < 0.001 by student’s t-test.

    Article Snippet: Following antibodies were purchased and used for immunostaining: CD68 (FA-11, Bio-Rad, MCA1957, 1:200), Actin, α-Smooth Muscle (1A4, Sigma-Aldrich, F3777, 1:500), CD31 (2H8, Merck Millipore, MAB1398Z, 1:200), CD31 (MEC13.3, BD Pharmingen, 550274, 1:400), Icam1 (YN1/1.7.4, BioLegend, 116101, 1:200), Chop (F-168, Santa Cruz, sc-575, 1:100), NF-κB (C-20, Santa Cruz, sc-372, 1:200) and Cleaved Caspase-3 (Asp175, Cell signaling technology, #9661, 1:200).

    Techniques: In Vivo, Staining